Research > Focus D > Work Packages D01
Summary
This central project aims at ensuring uniform availability and quality of tissue material and high-throughput data in cost-effective and state-of-technology way to be used in UNITE subprojects (coined UNITE Core Collection). Our aim is to work with and provide material and data from newly diagnosed patients and matching recurrences, which has been stringently standardized, characterized and anaylzed reflecting the multilevel approach of the CRC. The visual abstract below shows the aims and planned tasks of the work package.
Task 1
Tumor banking and clinical data management in Heidelberg and Mannheim
Steps / Workflow
- Obtaining patient consent
- Archiving tumor tissue
- Quality control
- Preparing tumor sections, RNA, DNA and proteins
- Assessing clinical data
Task 2
Preparation of cell isolates and primary cell cultures
Steps / Workflow
- Preparing single cell suspensions from tumor tissues for functional analyses, PDX models, primary cultures and further sorting of distinct cell populations
- Establishing primary cultures
Task 3
Molecular characterization of tumors and primary cell culture UNITE Core Collection
Steps / Workflow
- 100 newly diagnosed glioblastoma samples and 20 matching recurrences, for which we will establish an uniform data matrix, consisting of epigenome analysis, whole-genome sequencing, RNA sequencing, single cell sequencing, data on a set of currently relevant biomarkers, and metabolomics and proteomic profiling as well as fully characterized PDX of >30%
Task 4
Proteomics
Steps / Workflow
- Development and implementation of protocols for identification and quantification of proteins extracted from FFPE tissue
- Development and implementation of protocols for identification and quantification of protein modifications on fresh frozen tissue
Principle Investigators
VON DEIMLING, ANDREAS, PROF. DR. MED.
Chair / Head of Department
University Hospital Heidelberg, Institute of Pathology, Department of Neuropathology & DKFZ, Clinical Cooperation Unit Neuropathology, Im Neuenheimer Feld 224, 69120 Heidelberg, Germany
HEROLD-MENDE, CHRISTEL, PROF. DR. RER. NAT.
Professor for Experimental Neurosurgery, Division Head
University Hospital Heidelberg, Department of Neurosurgery, Division of Neurosurgical Research, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany
Ratliff, Miriam, Dr.med., Dipl.-Biol.
Constultant, Department of Neurosurgery
University Medical Centre Mannheim, Medical Faculty Mannheim, Heidelberg University Theodor-Kutzer-Ufer 1-3, 68167 Mannheim